Effects of the polyubiquitin gene Ubi.U4 leader intron and first ubiquitin monomer on reporter gene expression in Nicotiana tabacum

被引:54
作者
Plesse, B [1 ]
Criqui, MC [1 ]
Durr, A [1 ]
Parmentier, Y [1 ]
Fleck, J [1 ]
Genschik, P [1 ]
机构
[1] CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France
关键词
cell division; gene expression; intron; tobacco; ubiquitin;
D O I
10.1023/A:1010671405594
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously shown by RNA gel blot analyses that the tobacco polyubiquitin-encoding gene Ubi.U4 is expressed in a complex pattern during plant development (Genschik et al., 1994). In order to study its tissue-specific expression, we cloned the fragment containing the -263 bp proximal promoter of the gene, the leader intron and the first ubiquitin monomer in front of the reporter GUS gene. Histochemical analyses for GUS activity during tobacco plant development revealed that the gene is expressed at variable amounts in many plant tissues with high levels in metabolically active and/or dividing cells and in the vascular tissues of the plant. We also analysed the expression pattern of constructs in which either the intron or the intron together with the first ubiquitin monomer were deleted. Our results indicate that the ubiquitin leader intron is not only a quantitative determinant of gene expression but may also influence the tissue-specific expression pattern.
引用
收藏
页码:655 / 667
页数:13
相关论文
共 74 条
[1]   DIFFERENTIAL ACCUMULATION OF SUNFLOWER TETRAUBIQUITIN MESSENGER-RNAS DURING ZYGOTIC EMBRYOGENESIS AND DEVELOPMENTAL REGULATION OF THEIR HEAT-SHOCK RESPONSE [J].
ALMOGUERA, C ;
COCA, MA ;
JORDANO, J .
PLANT PHYSIOLOGY, 1995, 107 (03) :765-773
[2]   ALTERED LIGNIN COMPOSITION IN TRANSGENIC TOBACCO EXPRESSING O-METHYLTRANSFERASE SEQUENCES IN SENSE AND ANTISENSE ORIENTATION [J].
ATANASSOVA, R ;
FAVET, N ;
MARTZ, F ;
CHABBERT, B ;
TOLLIER, MT ;
MONTIES, B ;
FRITIG, B ;
LEGRAND, M .
PLANT JOURNAL, 1995, 8 (04) :465-477
[3]   PERTURBATION OF THE UBIQUITIN SYSTEM CAUSES LEAF CURLING, VASCULAR TISSUE ALTERATIONS AND NECROTIC LESIONS IN A HIGHER-PLANT [J].
BACHMAIR, A ;
BECKER, F ;
MASTERSON, RV ;
SCHELL, J .
EMBO JOURNAL, 1990, 9 (13) :4543-4549
[4]  
Belknap WR, 1996, TRENDS PLANT SCI, V1, P331
[5]   STRUCTURE AND EXPRESSION OF SUNFLOWER UBIQUITIN GENES [J].
BINET, MN ;
WEIL, JH ;
TESSIER, LH .
PLANT MOLECULAR BIOLOGY, 1991, 17 (03) :395-407
[6]  
BOLLE C, 1994, PLANT J, V6, P213
[7]   UBIQUITIN FUSION AUGMENTS THE YIELD OF CLONED GENE-PRODUCTS IN ESCHERICHIA-COLI [J].
BUTT, TR ;
JONNALAGADDA, S ;
MONIA, BP ;
STERNBERG, EJ ;
MARSH, JA ;
STADEL, JM ;
ECKER, DJ ;
CROOKE, ST .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2540-2544
[8]   DEVELOPMENTALLY-REGULATED LOSS OF UBIQUITIN AND UBIQUITINATED PROTEINS DURING POLLEN MATURATION IN MAIZE [J].
CALLIS, J ;
BEDINGER, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :6074-6077
[9]  
CALLIS J, 1990, J BIOL CHEM, V265, P12486
[10]  
CALLIS J, 1995, GENETICS, V139, P921