PCR for specific detection of H7 flagellar variant of fliC among extraintestinal pathogenic Escherichia coli

被引：11

作者：

Johnson, JR

Stell, AL

机构：

[1] Minneapolis VA Med Ctr, Med Serv, Minneapolis, MN 55417 USA

[2] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期

关键词：

D O I：

10.1128/JCM.39.10.3712-3717.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A newly developed PCR-based assay for the H7 variant of the Escherichia coli flagellin gene, fliC, was 100% sensitive and specific in comparison with serology and probe hybridization. It revealed broad conservation of the H7 fliC variant among phylogenetically diverse lineages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7 serotyping results. The H7 primers functioned well when incorporated into a multiplex PCR assay for diverse virulence-associated genes of ExPEC.

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收藏

页码：3712 / 3717

页数：6

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