Hygromycin resistance as an efficient selectable marker for wheat stable transformation

被引：52

作者：

Ortiz, JPA ^{[1
]}

Reggiardo, MI ^{[1
]}

Ravizzini, RA ^{[1
]}

Altabe, SG ^{[1
]}

Cervigni, GDL ^{[1
]}

Spitteler, MA ^{[1
]}

Morata, MM ^{[1
]}

Elias, FE ^{[1
]}

Vallejos, RH ^{[1
]}

机构：

[1] UNIV NACL ROSARIO,CONICET,CEFOBI,RA-2000 ROSARIO,ARGENTINA

来源：

PLANT CELL REPORTS | 1996年 / 15卷 / 12期

关键词：

transgenic wheat; hygromycin selection; microparticle accelerator; PCR amplification of transgenes;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A highly efficient method for stable wheat transformation using hygromycin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder-driven microparticle accelerator. Transgenic wheat plants were determined by PCR amplification of transgene fragments and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relative to the number of bombarded calli) of 5.5% compared with 2.6% for the bar gene.

引用

页码：877 / 881

页数：5

共 19 条

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