Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR test and manual microwell plate-based AMPLICOR HBV MONITOR test

被引：14

作者：

Marin, IJ

Poljak, M

Seme, K

Meglic-Volikar, J

Maticic, M

Lesnicar, G

Brinovec, V

机构：

[1] Med Fac Ljubljana, Inst Microbiol & Immunol, Ljubljana 1105, Slovenia

[2] Ctr Clin, Dept Infect Dis, Ljubljana 1105, Slovenia

[3] Gen Hosp, Dept Infect Dis, Celje, Slovenia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 02期

关键词：

D O I：

10.1128/JCM.39.2.758-761.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV DNA positive by both assays. Three samples tested positive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related (n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.

引用

收藏

页码：758 / 761

页数：4

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