Oxidative DNA damage in patients with cataract

被引：35

作者：

Ates, Orhan ^{[1
]}

Alp, Hakan Hamit ^{[2
]}

Kocer, Ibrahim

Baykal, Orhan

Salman, Ilknur A.

机构：

[1] Ataturk Univ, Tip Fak, Dept Ophthalmol, Goz Hastaliklari ABD,Med Fac, TR-25240 Erzurum, Turkey

[2] Ataturk Univ, Dept Biochem, Fac Med, TR-25240 Erzurum, Turkey

来源：

ACTA OPHTHALMOLOGICA | 2010年 / 88卷 / 08期

关键词：

cataract; malondialdehyde; oxidative DNA damage; oxidative stress; DIABETIC-RETINOPATHY; LIPID-PEROXIDATION; STRESS; MALONDIALDEHYDE; BIOMARKERS; LENS; 8-HYDROXY-2'-DEOXYGUANOSINE; 8-HYDROXYDEOXYGUANOSINE; CARCINOGENESIS; PATHOGENESIS;

D O I：

10.1111/j.1755-3768.2009.01583.x

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: This study examines the levels of oxidative damage in patients with cataract. Methods: Blood samples were collected from 60 patients with cataract and 60 age- and gender-matched healthy individuals to measure 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels. Results: A significant difference was observed in leukocyte 8-OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p < 0.001). In addition, a significant correlation was found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r = 0.859, p < 0.001). Conclusion: This study measured the oxidative DNA damage by measuring the 8-OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA - a marker for lipid peroxidation - was measured to determine lipid peroxidation.

引用

页码：891 / 895

页数：5

共 43 条

[1]

Alberti G, 1996, INVEST OPHTH VIS SCI, V37, P1167

[2]

ALIO JL, 1995, CORNEA, V14, P175

[3] Plasma catalase activity and malondialdehyde level in patients with cataract [J].

Ates, NA ;

Yildirim, Ö ;

Tamer, L ;

Ünlü, A ;

Ercan, B ;

Muslu, N ;

Kanik, A ;

Hatungil, R ;

Atik, U .

EYE, 2004, 18 (08) :785-788

[4] LIPID PEROXIDE AND REACTIVE OXYGEN SPECIES GENERATING SYSTEMS OF THE CRYSTALLINE LENS [J].

BABIZHAYEV, MA ;

COSTA, EB .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1994, 1225 (03) :326-337

[5] Lipid peroxidation and cataracts: N-Acetylcarnosine as a therapeutic tool to manage age-related cataracts in human and in canine eyes [J].

Babizhayev M.A. ;

Deyev A.I. ;

Yermakova V.N. ;

Brikman I.V. ;

Bours J. .

Drugs in R & D, 2004, 5 (3) :125-139

[6] The role of oxidative stress in the pathogenesis of age-related macular degeneration [J].

Beatty, S ;

Koh, HH ;

Henson, D ;

Boulton, M .

SURVEY OF OPHTHALMOLOGY, 2000, 45 (02) :115-134

[7] Role of malondialdehyde and superoxide dismutase in cataractogenesis [J].

Bhatia, R. P. ;

Rai, Ruchi ;

Rao, G. R. K. .

ANNALS OF OPHTHALMOLOGY, 2006, 38 (02) :103-106

[8]

Bonnefoy M, 2002, PRESSE MED, V31, P1174

[9] Relationship between two oxidative stress biomarkers, malondialdehyde and 8-oxo-7,8-dihydro-2′-deoxyguanosine, in the freshwater bivalve Unio tumidus [J].

Charissou, AM ;

Cossu-Leguille, C ;

Vasseur, P .

SCIENCE OF THE TOTAL ENVIRONMENT, 2004, 322 (1-3) :109-122

[10] LENS OPACITIES CLASSIFICATION SYSTEM-II (LOCS-II) [J].

CHYLACK, LT ;

LESKE, MC ;

MCCARTHY, D ;

KHU, P ;

KASHIWAGI, T ;

SPERDUTO, R .

ARCHIVES OF OPHTHALMOLOGY, 1989, 107 (07) :991-997

← 1 2 3 4 5 →