Antibacterial characteristics and activity of acid-soluble chitosan

被引：355

作者：

Chung, Ying-Chien ^{[1
]}

Chen, Chih-Yu ^{[2
]}

机构：

[1] China Inst Technol, Dept Biol Sci & Technol, Taipei 115, Taiwan

[2] Hsing Wu Coll, Dept Tourism, Taipei 224, Taiwan

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 08期

关键词：

chitosan; antibacterial action; bactericide; antibacterial activity;

D O I：

10.1016/j.biortech.2007.06.044

中图分类号：

S2 [农业工程];

学科分类号：

0828 [农业工程];

摘要：

The antibacterial activity of chitosan was investigated by assessing the mortality rates of Escherichia coli and Staphylococcus aureus based on the extent of damaged or missing cell walls and the degree of leakage of enzymes and nucleotides from different cellular locations. Chitosan was found to react with both the cell wall and the cell membrane, but not simultaneously, indicating that the inactivation of E coli by chitosan occurs via a two-step sequential mechanism: an initial separation of the cell wall from its cell membrane, followed by destruction of the cell membrane. The similarity between the antibacterial profiles And patterns of chitosan and those of two control substances, polymyxin and EDTA, verified this mechanism. The antibacterial activity of chitosan could be altered by blocking the amino functionality through coupling of the chitosan to active agarose derivatives. These results verify the status of chitosan as a natural bactericide. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：2806 / 2814

页数：9

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