LRH-1/hB1F and HNF1 synergistically up-regulate hepatitis B virus gene transcription and DNA replication

被引：37

作者：

Cai, YN

Zhou, Q

Kong, YY

Li, M

Viollet, B

Xie, YH

Wang, Y

机构：

[1] Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China

[2] Inst Pasteur, Dept Biotechnol, Oncogen Viruses Unit, Paris, France

来源：

CELL RESEARCH | 2003年 / 13卷 / 06期

关键词：

LRH-1/hB1F; HNF1; HBV; ENII; synergism;

D O I：

10.1038/sj.cr.7290187

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Enhancer II (ENII) is one of the critical cis-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hB1F, HNF1, HNF3beta, HNF4 and C/EBP. Knowledge on the interplay of these important factors is still limited. In this study, we demonstrate a functional synergism between the orphan nuclear receptor LRH-1/hB1F and the homeoprotein HNF1 in up-regulating the liver-specific activity of ENII. This synergism is sufficient for initiating the viral gene transcription and DNA replication in non-hepatic cells. We have defined the activation domains in hB1F and HNF1 that contribute to the synergism. We further show that hB1F and HNF1 can interact directly in vitro and have mapped the domains required for this interaction.

引用

页码：451 / 458

页数：8

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