The srhSR gene pair from Staphylococcus aureus:: Genomic and proteomic approaches to the identification and characterization of gene function

被引:83
作者
Throup, JP [1 ]
Zappacosta, F
Lunsford, RD
Annan, RS
Carr, SA
Lonsdale, JT
Bryant, AP
McDevitt, D
Rosenberg, M
Burnham, MKR
机构
[1] GlaxoSmithKline Pharmaceut Res & Dev, Antiinfect Res, Collegeville, PA 19426 USA
[2] GlaxoSmithKline, Dept Phys & Struct Chem, King Of Prussia, PA 19406 USA
关键词
D O I
10.1021/bi0102959
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Systematic analysis of the entire two-component signal transduction system (TCSTS) gene complement of Staphylococcus aureus revealed the presence of a putative TCSTS (designated SrhSR) which shares considerable homology with the ResDE His-Asp phospho-relay pair of Bacillus subtilis. Disruption of the srhSR gene pair resulted in a dramatic reduction in growth of the srhSR mutant, when cultured under anaerobic conditions, and a 3-log attenuation in growth when analyzed in the murine pyelonephritis model. To further understand the role of SrhSR, differential display two-dimensional gel electrophoresis was used to analyze the cell-free extracts derived from the srhSR mutant and the corresponding wild type. Proteins shown to be differentially regulated were identified by mass spectrometry in combination with protein database searching. An srhSR deletion led to changes in the expression of proteins involved in energy metabolism and other metabolic processes including arginine catabolism, xanthine catabolism, and cell morphology. The impaired growth of the mutant under anaerobic conditions and the dramatic changes in proteins involved in energy metabolism shed light on the mechanisms used by S. aureus to grow anaerobically and indicate that the staphylococcal SrhSR system plays an important role in the regulation of energy transduction in response to changes in oxygen availability. The combination of proteomics, bio-informatics, and microbial genetics employed here represents a powerful set of techniques which can be applied to the study of bacterial gene function.
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页码:10392 / 10401
页数:10
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