Macromolecular crowding and confinement: Biochemical, biophysical, and potential physiological consequences

被引：1673

作者：

Zhou, Huan-Xiang ^{[1
,2
]}

Rivas, German ^{[3
]}

Minton, Allen P. ^{[4
]}

机构：

[1] Florida State Univ, Dept Phys, Sch Computat Sci, Tallahassee, FL 32306 USA

[2] Florida State Univ, Dept Phys, Inst Mol Biophys, Tallahassee, FL 32306 USA

[3] CSIC, Ctr Invest Biol, Dept Ciencia Prot, E-28040 Madrid, Spain

[4] NIDDK, Sect Phys Biochem, Lab Biochem & Genet, NIH,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS | 2008年 / 37卷

关键词：

excluded volume; configurational entropy; free energy; protein-protein interactions; protein folding; site-binding;

D O I：

10.1146/annurev.biophys.37.032807.125817

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Expected and observed effects of volume exclusion on the free energy of rigid and flexible macromolecules in crowded and confined systems, and consequent effects of crowding and confinement on macromolecular reaction rates and equilibria are summarized. Findings from relevant theoretical/simulation and experimental literature published from 2004 onward are reviewed. Additional complexity arising from the heterogeneity of local environments in biological media, and the presence of nonspecific interactions between macromolecules over and above steric repulsion, are discussed. Theoretical and experimental approaches to the characterization of crowding and confinement-induced effects in systems approaching the complexity of living organisms are suggested.

引用

页码：375 / 397

页数：23

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