Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases

被引：1917

作者：

Whitmore, Lee ^{[1
]}

Wallace, B. A. ^{[1
]}

机构：

[1] Univ London, Birkbeck Coll, Dept Crystallog, London WC1E 7HX, England

来源：

BIOPOLYMERS | 2008年 / 89卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

circular dichroism spectroscopy; protein secondary structure; analyses; reference database; bioinformatics; synchrotron radiation circular dichroism (SRCD);

D O I：

10.1002/bip.20853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein secondary structures for many years. With the advent of synchrotron radiation circular dichroism (SRCD) and improvements in instrumentation for conventional CD, lower wavelength data are obtainable and the information content of the spectra increased. In addition, new computation and bioinformatics methods have been developed and new reference databases have been created, which greatly improve and facilitate the analyses of CD spectra. This article discusses recent developments in the analysis of protein secondary structures, including features of the DICHROWEB analysis webserver. (c) 2007 Wiley Periodicals, Inc.

引用

页码：392 / 400

页数：9

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