Mechanical stretch is a down-regulatory signal for differentiation of C-2C12 myogenic cells

被引：28

作者：

Akimoto, T

Ushida, T

Miyaki, S

Tateishi, T

Fukubayashi, T

机构：

[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Tokyo 1538902, Japan

[2] AIST, Natl Inst Adv Ind Sci & Technol, Tissue Engn Res Ctr, Tsukuba, Ibaraki 3058562, Japan

[3] Univ Tokyo, Grad Sch Engn, Biomed Engn Lab, Tokyo 1138656, Japan

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2001年 / 17卷 / 1-2期

关键词：

mechanical stretch; myogenic cells; myogenic regulatory factors;

D O I：

10.1016/S0928-4931(01)00340-X

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The purpose of this study was to examine whether mechanical stretch alters the expression of myogenic regulatory factors in cultured myogenic satellite cells. C2Cl2 myogenic satellite cells were plated on collagenized silastic membrane and cultured in growth medium and stretched with 20% elongation for 24 h. The expressions of myogenic regulatory factors including MyoD, myogenin, myocyte nuclear factor (MNF)-alpha and -beta were determined by semiquantitive reverse transcriptional-polymerase chain reaction (RT-PCR) analysis, The expressions of MyoD and MNF-alpha were decreased in stretched C2Cl2 as compared to control condition. The expressions of myogenin and MNF-beta were not detected. These results demonstrate that mechanical stretch inhibits differentiation of C2Cl2 myogenic satellite cells to myotube in the cultured condition. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：75 / 78

页数：4

共 10 条

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