Maize genome sequencing by methylation filtrations

被引：159

作者：

Palmer, LE ^{[1
]}

Rabinowicz, PD ^{[1
]}

O'Shaughnessy, AL ^{[1
]}

Balija, VS ^{[1
]}

Nascimento, LU ^{[1
]}

Dike, S ^{[1
]}

de la Bastide, M ^{[1
]}

Martienssen, RA ^{[1
]}

McCombie, WR ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

来源：

SCIENCE | 2003年 / 302卷 / 5653期

关键词：

D O I：

10.1126/science.1091265

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.

引用

页码：2115 / 2117

页数：3

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