The mouse poly (C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins

被引:39
作者
Funke, B
Zuleger, B
Benavente, R
Schuster, T
Goller, M
Stevenin, J
Horak, I
机构
[1] FORSCH INST MOL PHARMAKOL,D-12207 BERLIN,GERMANY
[2] UNIV WURZBURG,INST IMMUNOL & VIROL,WURZBURG,GERMANY
[3] THEODOR BOVERI INST BIOWISSENSCH,LEHRSTUHL ZELL & ENTWICKLUNGSBIOL,WURZBURG,GERMANY
[4] UNIV WURZBURG,INST MED STRAHLENKUNDE & ZELLFORSCH,WURZBURG,GERMANY
[5] CNRS,INST GENET & BIOL MOL & CELLULAIRE,STRASBOURG,FRANCE
关键词
D O I
10.1093/nar/24.19.3821
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The murine poly(C)-binding protein (mCBP) was previously shown to belong to the group of K-homology(KH) proteins by virtue of its homology to hnRNP-K. We have isolated cDNA-splice variants of mCBP which differ by two variable regions of 93 bp and/or 39 +/- 3 bp respectively. Both variable regions are located between the second and third KH-domain of mCBP. The characterization of a partial genomic clone enabled us to propose a model for the generation of the second variable region by the use of a putative alternative spl ice signal. The mCBP mRNA is expressed ubiquitously and the protein is found predominantly in the nucleus with the exception of the nucleoli. We have identified five proteins which interact with mCBP in the yeast two hybrid system: mouse y-box protein 1 (msy-1), y-box-binding protein, hnRNP-L, filamin and splicing factor 9G8. The interaction between mCBP and splicing factor 9G8 was confirmed in vivo. These results suggest a function of mCBP in RNA metabolism.
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页码:3821 / 3828
页数:8
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