Evaluation of a tetrazolium salt test to determine absence of live mycobacteria in tuberculin purified protein derivative

被引：3

作者：

Bedwell, J ^{[1
]}

Kairo, SK ^{[1
]}

Corbel, MJ ^{[1
]}

机构：

[1] Natl Inst Biol Stand & Controls, Div Bacteriol, Potters Bar EN6 3QG, Herts, England

来源：

BIOLOGICALS | 2001年 / 29卷 / 01期

关键词：

D O I：

10.1006/biol.2001.0267

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current methodology to determine absence of live mycobacteria in tuberculin purified protein derivative (PPD) takes up to 8 weeks to perform and may also involve testing on animals. In this paper we describe an in vitro test utilising the tetrazolium salt, 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) to monitor the absence of live Mycobacterium tuberculosis (Mtb) in PPD. In the presence of live cells XTT is converted to a coloured formazan product that can be measured spectrophotometrically. Live mycobacteria present in spiked PPD were detected by a marked change in optical density above background levels. This; test is easy to perform and is complete in just 48 hr.

引用

页码：3 / 6

页数：4

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