Development of RNA aptamers for detection of Salmonella Enteritidis

被引：39

作者：

Hyeon, Ji-Yeon ^{[1
]}

Chon, Jung-Whan ^{[1
]}

Choi, In-Soo ^{[2
]}

Park, Chankyu ^{[3
]}

Kim, Dong-Eun ^{[4
]}

Seo, Kun-Ho ^{[1
]}

机构：

[1] Konkuk Univ, KU Ctr Food Safety, Seoul 143701, South Korea

[2] Konkuk Univ, Dept Infect Dis, Coll Vet Med, Seoul 143701, South Korea

[3] Konkuk Univ, Dept Anim Biotechnol, Coll Anim Biosci & Technol, Seoul 143701, South Korea

[4] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 89卷 / 01期

关键词：

Salmonella Enteritidis; RNA aptamer; SELEX; Detection; FIELD GEL-ELECTROPHORESIS; ULTRASENSITIVE DETECTION; MAGNETIC BEAD; DNA APTAMERS; ASSAY; SELECTION; EGGS; PCR;

D O I：

10.1016/j.mimet.2012.01.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed and evaluated RNA aptamers to analyze their potential for use in detecting Salmonella Enteritidis. The selected aptamer was observed to specifically bind to Salmonella Enteritidis without any cross-reactivity to other Salmonella serovars. Thus, this study suggests that aptamers specific to Salmonella Enteritidis have a high potential for use in presumptive presumptive screening methods or alternative serotyping methods. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：79 / 82

页数：4

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