The β-amyloid-related proteins presenilin 1 and BACE1 are axonally transported to nerve terminals in the brain

被引：50

作者：

Sheng, JG

Price, DL

Koliatsos, VE

机构：

[1] Johns Hopkins Univ, Sch Med, Div Neuropathol, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Alzheimers Dis Res Ctr, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

来源：

EXPERIMENTAL NEUROLOGY | 2003年 / 184卷 / 02期

关键词：

hippocampus; perforant pathway; amyloid; Alzheimer disease; axonal transport; beta-secretase; APP;

D O I：

10.1016/j.expneurol.2003.08.018

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In this study, we show that removal of entorhinal cortex (ERC) afferents to hippocampus reduces levels of presenilin I (PSI) in the dentate gyrus of APPswe/PS1DeltaE9 transgenic (Tg) mice. PS1 immunoreactivity on the deafferented dentate gyrus decreases by approximately 25% and 50%, 2 and 4 weeks post-lesion compared to the contralateral side; by Western blotting, there is an approximately 40% decrease of the 43 kDa (full length) PSI and an approximately 80% decrease of the 28 kDa (N-terminal fragment) PSI on the lesioned dentate gyrus. Levels of beta-site APP Cleavage Enzyme 1 (BACE1) immunoreactivity also decrease by approximately 50% and 65% 2 and 4 weeks post-lesion. Together, these data demonstrate that PSI and BACE I are transported from the entorhinal cortex to the hippocampus via axons of the perforant pathway. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：1053 / 1057

页数：5

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