An upstream repressor element plays a role in Igf2 imprinting

被引：79

作者：

Eden, S

Constancia, M

Hashimshony, T

Dean, W

Goldstein, B

Johnson, AC

Keshet, I

Reik, W

Cedar, H

机构：

[1] Hadassah Univ Hosp, Dept Cellular Biochem, IL-91120 Jerusalem, Israel

[2] Babraham Inst, Inst Grassland & Anim Prod, Cambridge CB2 4AT, England

[3] NCI, Canc Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

EMBO JOURNAL | 2001年 / 20卷 / 13期

关键词：

GCF; imprinting; methylation;

D O I：

10.1093/emboj/20.13.3518

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele, We have identified a repressor element within this sequence and shown that repression is probably mediated through a transacting factor, GCF2, DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.

引用

页码：3518 / 3525

页数：8

共 38 条

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