Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts

被引：192

作者：

Dash, Rupesh ^{[1
]}

Acharya, Chitrangada ^{[1
]}

Bindu, P. C. ^{[1
]}

Kundu, S. C. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

BMB REPORTS | 2008年 / 41卷 / 03期

关键词：

antioxidant; fibroblasts; hydrogen peroxide; oxidative stress; silk protein sericin;

D O I：

10.5483/BMBRep.2008.41.3.236

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The antioxidant potential of silk protein sericin from the non-mulberry tropical tasar silkworm Antheraea mylitta cocoon has been assessed and compared with that of the mulberry silkworm, Bombyx mori. Skin fibroblast cell line (AH927) challenged with hydrogen peroxide served as the positive control for the experiment Our results showed that the sericin obtained from tasar cocoons offers protection against oxidative stress and cell viability is restored to that of control on pre-incubation with the sericin. Fibroblasts pre-incubated with non-mulberry sericin had significantly lower levels of catalase, lactate dehydrogenase and malondialdehyde activity when compared to untreated ones. This report indicates that the silk protein sericin from the non-mulberry tropical tasar silkworm, A. mylitta can serve as a valuable antioxidant.

引用

页码：236 / 241

页数：6

共 38 条

[1]

MECHANISMS FOR REGULATING OXYGEN-TOXICITY IN PHYTOPHAGOUS INSECTS [J].

AHMAD, S ;

PARDINI, RS .

FREE RADICAL BIOLOGY AND MEDICINE, 1990, 8 (04) :401-413

[2]

Silk-based biomaterials [J].

Altman, GH ;

Diaz, F ;

Jakuba, C ;

Calabro, T ;

Horan, RL ;

Chen, JS ;

Lu, H ;

Richmond, J ;

Kaplan, DL .

BIOMATERIALS, 2003, 24 (03) :401-416

[3]

Arora S, 2003, TERATOGEN CARCIN MUT, P295

[4]

Bergmeyer H-U, 1963, METHOD ENZYMAT AN, P574

[5]

SENESCENCE-LIKE GROWTH ARREST INDUCED BY HYDROGEN-PEROXIDE IN HUMAN-DIPLOID FIBROBLAST F65 CELLS [J].

CHEN, Q ;

AMES, BN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (10) :4130-4134

[6]

TOPICAL VITAMIN-C PROTECTS PORCINE SKIN FROM ULTRAVIOLET RADIATION-INDUCED DAMAGE [J].

DARR, D ;

COMBS, S ;

DUNSTON, S ;

MANNING, T ;

PINNELL, S .

BRITISH JOURNAL OF DERMATOLOGY, 1992, 127 (03) :247-253

[7]

Purification and biochemical characterization of a 70 kDa sericin from tropical tasar silkworm, Antheraea mylitta [J].

Dash, Rupesh ;

Ghosh, Sudip K. ;

Kaplan, David L. ;

Kundu, S. C. .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2007, 147 (01) :129-134

[8]

Isolation, purification and characterization of silk protein sericin from cocoon peduncles of tropical tasar silkworm, Antheraea mylitta [J].

Dash, Rupesh ;

Mukherjee, Soumen ;

Kundu, S. C. .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2006, 38 (3-5) :255-258

[9]

p53 dependent apoptosis in glioma cell lines in response to hydrogen peroxide induced oxidative stress [J].

Datta, K ;

Babbar, P ;

Srivastava, T ;

Sinha, S ;

Chattopadhyay, P .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2002, 34 (02) :148-157

[10]

Lipid peroxidation induced by bolesatine, a toxin of Boletus satanas: Implication in m(5)dC variation in Vero cells related to inhibition of cell growth [J].

Ennamany, R ;

Marzetto, S ;

Saboureau, D ;

Creppy, EE .

CELL BIOLOGY AND TOXICOLOGY, 1995, 11 (06) :347-354

← 1 2 3 4 →