Immunological properties of umbilical cord blood-derived mesenchymal stromal cells

被引：108

作者：

Oh, Wonil ^{[2
]}

Kim, Dal-Soo ^{[2
]}

Yang, Yoon Sun ^{[2
]}

Lee, Jae Kwon ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Educ, Dept Sci Educ Biol, Chungcheongbuk Do 361763, South Korea

[2] Medipost Co Ltd, Asan Inst Life Sci, Medipost Biomed Res Inst, Seoul 138736, South Korea

来源：

CELLULAR IMMUNOLOGY | 2008年 / 251卷 / 02期

关键词：

mesenchymal stromal cells; umbilical cord blood; allogeneic response; cell therapy; interferon-gamma; mixed lymphocyte reaction;

D O I：

10.1016/j.cellimm.2008.04.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mesenchymal stromal cells (MSCs) are promising candidates for developing cell therapies for intractable diseases. To assess the feasibility of transplantation with human umbilical cord blood (hUCB)-derived MSCs, we analyzed the ability of these cells to function as alloantigen-presenting cells (APC) in vitro. hUCB-MSCs were strongly positive for MSC-related antigens and stained positively for human leukocyte antigen (HLA)-AB and negatively for HLA-DR. When treated with interferon (IFN)-gamma, the expression of HLA-AB and HLA-DR, but not the co-stimulatory molecules CD80 and CD86, was increased. hUCB-MSCs did not provoke allogeneic PBMC (peripheral blood mononuclear cell) proliferation, even when their HLA-molecule expression was up-regulated by IFN-gamma pretreatment. When added to a mixed lymphocyte reaction (MLR), hUCB-MSCs actively suppressed the allogeneic proliferation of the responder lymphocytes. This suppressive effect was mediated by soluble factors. We conclude that hUCB-MSCs can suppress the allogeneic response of lymphocytes and may thus be useful in allogeneic cell therapies. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：116 / 123

页数：8

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