Activation of p44/p42 mitogen-activated protein kinase limits triiodothyronine-stimulated alkaline phosphatase activity in osteoblasts

被引：13

作者：

Kozawa, O ^{[1
]}

Hatakeyama, D ^{[1
]}

Yoshida, M ^{[1
]}

Kamiya, Y ^{[1
]}

Kondo, C ^{[1
]}

Matsuno, H ^{[1
]}

Uematsu, T ^{[1
]}

机构：

[1] Gifu Univ, Sch Med, Dept Pharmacol, Gifu 5008705, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 286卷 / 05期

关键词：

triiodothyronine; alkaline phosphatase; MAP kinase; osteoblast;

D O I：

10.1006/bbrc.2001.5515

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been shown that thyroid hormone stimulates the activity of alkaline phosphatase, a marker of mature osteoblast phenotype, in osteoblasts. In the present study, we investigated whether p44/p42 mitogen-activated protein. (MAP) kinase is involved in the thyroid hormone-stimulated alkaline phosphatase activity in osteoblast-like MC3T3-E1 cells. Triiodothyronine (T-3) markedly induced the phosphorylation of p44/p42 MAP kinase. PD98059 and U0126, inhibitors of the upstream kinase that activates p44/p42 MAP kinase, significantly enhanced the T-3-induced alkaline phosphatase activity in a dose-dependent manner. The phosphorylation of p44/p42 MAP kinase induced by T-3 was reduced by U0126. These results strongly suggest that p44/p42 MAP kinase takes part in the thyroid hormone-stimulated alkaline phosphatase activity in. osteoblasts and that p44/p42 MAP kinase plays an inhibitory role in the thyroid hormone-effect. (C) 2001 Academic Press.

引用

页码：1140 / 1143

页数：4

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