Phenotypic and functional assessment of intraepithelial lymphocytes (IEL) isolated from rat colon and small bowel

被引：7

作者：

Gorczynski, RM ^{[1
]}

Chen, ZQ ^{[1
]}

Cohen, Z ^{[1
]}

Plapler, H ^{[1
]}

Wojcik, D ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT IMMUNOL,TORONTO,ON M5G 2C4,CANADA

来源：

IMMUNOLOGY LETTERS | 1996年 / 50卷 / 03期

基金：

英国医学研究理事会;

关键词：

intraepithelial lymphocytes; CD4:CD8; immunosuppresive factor;

D O I：

10.1016/0165-2478(96)02516-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have compared the proliferative potential of IELs isolated from rat colon (CIEL) and small bowel (SBIEL), and compared this with that observed using spleen lymphocytes. Unless additional irradiated spleen cells were added as a source of accessory cells, both IEL populations show poor proliferation in response to Con A stimulation. The CD4/CD8 ratio in spleen, SBIEL and CIEL was markedly different (3:1, 1:3, and 1:1, respectively). Cells expressing surface markers characteristic of macrophages were not routinely found in SBIELs. Both IEL preparations inhibited spleen cell proliferation in response to Con A or immobilized anti-CD3, and produced a soluble factor(s) capable of causing similar inhibition. For CIEL this inhibition was dependent upon a proliferation-independent but cell-cell contact dependent event.

引用

页码：131 / 137

页数：7

共 46 条

[1]

CAMERINI V, 1993, J IMMUNOL, V151, P1765

[2]

CUVELIER CA, 1992, CLIN EXP IMMUNOL, V90, P275

[3] LYMPHOKINE PRODUCTION BY MITOGEN AND ANTIGEN ACTIVATED MOUSE INTRAEPITHELIAL LYMPHOCYTES [J].