GPIHBP1 Is Responsible for the Entry of Lipoprotein Lipase into Capillaries

被引：304

作者：

Davies, Brandon S. J. ^{[1
]}

Beigneux, Anne P. ^{[1
]}

Barnes, Richard H., II ^{[1
]}

Tu, Yiping ^{[1
]}

Gin, Peter ^{[1
]}

Weinstein, Michael M. ^{[2
]}

Nobumori, Chika ^{[1
]}

Nyren, Rakel ^{[3
]}

Goldberg, Ira ^{[4
]}

Olivecrona, Gunilla ^{[3
]}

Bensadoun, Andre ^{[5
]}

Young, Stephen G. ^{[1
,2
]}

Fong, Loren G. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA

[3] Umea Univ, Dept Med Biosci Physiol Chem, S-90187 Umea, Sweden

[4] Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY 10032 USA

[5] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA

来源：

CELL METABOLISM | 2010年 / 12卷 / 01期

基金：

美国国家卫生研究院;

关键词：

GPI-ANCHORED PROTEINS; ADIPOSE-TISSUE; CELL-SURFACE; RECEPTOR; BINDING; CHYLOMICRONEMIA; PLASMA; MICE; GENE;

D O I：

10.1016/j.cmet.2010.04.016

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The lipolytic processing of triglyceride-rich lipoproteins by lipoprotein lipase (LPL) is the central event in plasma lipid metabolism, providing lipids for storage in adipose tissue and fuel for vital organs such as the heart. LPL is synthesized and secreted by myocytes and adipocytes, but then finds its way into the lumen of capillaries, where it hydrolyzes lipoprotein triglycerides. The mechanism by which LPL reaches the lumen of capillaries has remained an unresolved problem of plasma lipid metabolism. Here, we show that GPIHBP1 is responsible for the transport of LPL into capillaries. In Gpihbp1-deficient mice, LPL is mislocalized to the interstitial spaces surrounding myocytes and adipocytes. Also, we show that GPIHBP1 is located at the basolateral surface of capillary endothelial cells and actively transports LPL across endothelial cells. Our experiments define the function of GPIHBP1 in triglyceride metabolism and provide a mechanism for the transport of LPL into capillaries.

引用

页码：42 / 52

页数：11

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