Application of in vitro methods to study carbon uptake and transport by AM fungi

被引:32
作者
Douds, DD
Pfeffer, PE
Shachar-Hill, Y
机构
[1] ARS, USDA, ERRC, Wyndmoor, PA 19038 USA
[2] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA
关键词
arbuscular mycorrhiza; carbon uptake; in vitro culture; nuclear magnetic resonance;
D O I
10.1023/A:1026466802354
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Just as multi-compartmented root chambers have advantages over standard plastic pots for the study of nutrient uptake by arbuscular mycorrhizal [AM] fungi in soil, so the split-plate in vitro system has advantages over the standard dual culture system for the study of the physiology of AM fungi. We used the split-plate culture system of Ri T-DNA transformed Daucus carota L. roots and Glomus intraradices Schenck & Smith, in which only the fungus has access to the distal compartment, to study the ability of germ tubes and extraradical and intraradical hyphae to take up C-13-labeled substrates. Labeled substrates were added to one side of the plate divider and plates were incubated for 8 weeks while the fungus proliferated on the side from which the root was excluded. Tissues then were recovered from the plate and examined via NMR spectroscopy. Results showed that the morphological phases of the fungus differed in their ability to take up these substrates, most notably that intraradical hyphae take up hexose while extraradical hyphae cannot. In addition, NMR studies indicated that intraradical hyphae actively synthesized lipids while extraradical hyphae did not. These data show that eventual axenic culture of AM fungi is more than a matter of finding the proper substrate for growth. Genetic regulation must be overcome to make extraradical hyphae behave like intraradical hyphae in terms of C uptake and metabolism.
引用
收藏
页码:255 / 261
页数:7
相关论文
共 21 条
[1]   Carbon metabolism in spores of the arbuscular mycorrhizal fungus Glomus intraradices as revealed by nuclear magnetic resonance spectroscopy [J].
Bago, B ;
Pfeffer, PE ;
Douds, DD ;
Brouillette, J ;
Bécard, G ;
Shachar-Hill, Y .
PLANT PHYSIOLOGY, 1999, 121 (01) :263-271
[2]   EARLY EVENTS OF VESICULAR ARBUSCULAR MYCORRHIZA FORMATION ON RI T-DNA TRANSFORMED ROOTS [J].
BECARD, G ;
FORTIN, JA .
NEW PHYTOLOGIST, 1988, 108 (02) :211-218
[3]  
BECARD G, 1989, APPL ENVIRON MICROB, V55, P2320
[4]   THE EARLY SYNTHESIS OF RNA, PROTEIN, AND SOME ASSOCIATED METABOLIC EVENTS IN GERMINATING VESICULAR ARBUSCULAR MYCORRHIZAL FUNGAL SPORES OF GLOMUS-CALEDONIUS [J].
BEILBY, JP ;
KIDBY, DK .
CANADIAN JOURNAL OF MICROBIOLOGY, 1982, 28 (06) :623-628
[5]   QUANTIFICATION OF COMPARTMENTED METABOLIC FLUXES IN MAIZE ROOT-TIPS USING ISOTOPE DISTRIBUTION FROM C-13-LABELED OR C-14-LABELED GLUCOSE [J].
DIEUAIDENOUBHANI, M ;
RAFFARD, G ;
CANIONI, P ;
PRADET, A ;
RAYMOND, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (22) :13147-13159
[6]   SOLUBILIZATION OF GELLAN GELS BY CHELATION OF CATIONS [J].
DONER, LW ;
BECARD, G .
BIOTECHNOLOGY TECHNIQUES, 1991, 5 (01) :25-28
[7]   An improved procedure for root surface disinfection suitable for observations of proliferation of intraradical hyphae of arbuscular mycorrhizal fungus Glomus fistulosum [J].
Gryndler, M ;
Hrselova, H ;
Chvatalova, I .
FOLIA MICROBIOLOGICA, 1997, 42 (05) :489-494
[8]   LIMITED INDEPENDENT GROWTH OF A VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGUS INVITRO [J].
HEPPER, CM .
NEW PHYTOLOGIST, 1983, 93 (04) :537-542
[9]   LIPID AND FATTY-ACID PROFILES OF SOME VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI - CONTRIBUTION TO TAXONOMY [J].
JABAJIHARE, S .
MYCOLOGIA, 1988, 80 (05) :622-629
[10]   PHOSPHORUS DEPLETION AND PH DECREASE AT THE ROOT SOIL AND HYPHAE SOIL INTERFACES OF VA MYCORRHIZAL WHITE CLOVER FERTILIZED WITH AMMONIUM [J].
LI, XL ;
GEORGE, E ;
MARSCHNER, H .
NEW PHYTOLOGIST, 1991, 119 (03) :397-404