Modulating effect of Withania somnifera on TCA cycle enzymes and electron transport chain in azoxymethane-induced colon cancer in mice

被引：18

作者：

Muralikrishnan, Govidan ^{[2
]}

Amanullah, Safiullah ^{[3
]}

Basha, Mohamed I. ^{[4
]}

Dinda, Amit K. ^{[2
]}

Shakeel, Faiyaz ^{[1
]}

机构：

[1] Al Arab Med Univ, Fac Pharm, Dept Pharmaceut, Benghazi 5341, Libya

[2] All India Inst Med Sci, Dept Pathol, New Delhi 110029, India

[3] Al Arab Med Univ, Fac Med, Dept Biochem, Benghazi 5341, Libya

[4] Al Arab Med Univ, Fac Med, Dept Anat, Benghazi 5341, Libya

来源：

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | 2010年 / 32卷 / 03期

关键词：

Azoxymethane; colon cancer; electron transport chain complexes; TCA cycle enzymes; withania somnifera; ABERRANT CRYPT FOCI; MOUSE COLON; TUMORS; SUSCEPTIBILITY; MITOCHONDRIA; MUTATIONS; DYSPLASIA; LESIONS;

D O I：

10.3109/08923970903581540

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

The aim of the present investigation was to evaluate the efficacy of Withania somnifera on tricarboxylic acid (TCA) cycle enzymes and electron transport chain in azoxymethane-induced experimental colon cancer in mice. Azoxymethane at the dose of 15 mg/kg body weight was induced intraperitoneally once in a week for 28 days. The progression in colon tumor development was correlated with the appearance of the histological biomarker and aberrant crypt foci (ACF). Azoxymethane-induced colon cancer animals were treated with 400 mg/kg body weight of W. somnifera once in a week orally for 28 days. After the experimental period, the animals were killed and analyzed for TCA cycle key enzymes, such as isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), and alpha-keto glutarate dehydrogenase (alpha-KGDH). The modulating effect of W. somnifera on TCA cycle key enzymes and electron transport chain complexes were investigated against colon cancer induced by azoxymethane in Swiss albino mice. Decreased activities of TCA cycle key enzymes such as ICDH, SDH, MDH, and a-KGDH in colon cancer bearing animals were observed. W. somnifera administration normalized these enzyme levels in azoxymethane-induced experimental mice. These results suggested that W. somnifera is the promising chemotherapeutic agent for the treatment of colon cancer.

引用

页码：523 / 527

页数：5

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