Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species

被引：50

作者：

Cannio, R ^{[1
]}

Fiorentino, G ^{[1
]}

Carpinelli, P ^{[1
]}

Rossi, M ^{[1
]}

Bartolucci, S ^{[1
]}

机构：

[1] CNR,IST BIOCHIM PROT & ENZIMOL,I-80125 NAPLES,ITALY

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 01期

关键词：

D O I：

10.1128/jb.178.1.301-305.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene adh encoding a NAD-dependent alcohol dehydrogenase from the novel strain RC3 of Sulfolobus sp. was cloned and sequenced. Both the adh gene from Sulfolobus sp. strain RC3 and the alcohol dehydrogenase gene from Sulfolobas solfataricus (DSM 1617) were expressed at a high level in Escherichia coli, and the recombinant enzymes were purified, characterized, and compared. Only a few amino acid replacements were responsible for the different kinetic and physicochemical features investigated.

引用

页码：301 / 305

页数：5

共 22 条

[1] SUBSTRATE-SPECIFICITY AND STEREOSELECTIVITY OF HORSE LIVER ALCOHOL-DEHYDROGENASE - KINETIC EVALUATION OF BINDING AND ACTIVATION PARAMETERS CONTROLLING THE CATALYTIC CYCLES OF UNBRANCHED, ACYCLIC SECONDARY ALCOHOLS AND KETONES AS SUBSTRATES OF THE NATIVE AND ACTIVE-SITE-SPECIFIC CO(II)-SUBSTITUTED ENZYME [J].