Matrix-induced inhibition of membrane binding contributes to human immunodeficiency virus type 1 particle assembly defects in murine cells

被引:25
作者
Hatziioannou, T
Martin-Serrano, J
Zang, T
Bieniasz, PD
机构
[1] Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA
[2] Rockefeller Univ, New York, NY 10021 USA
关键词
D O I
10.1128/JVI.79.24.15586-15589.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Defective human immunodeficiency virus type 1 (HIV-1) assembly in murine cells is accompanied by poor plasma membrane binding and proteolytic processing of the HIV-1 Gag precursor. Here, we show that such defects are induced by the propensity of the HIV-1 MA globular head to inhibit membrane binding and particle assembly, particularly at the low expression levels observed in murine cells. Simple additions to or deletion of the MA globular head can improve the yield of infectious virions from murine cells by > 50-fold. Expression level and autoinhibition can be important confounding variables in studies of HIV-1 assembly and contribute to defects encountered in murine cells.
引用
收藏
页码:15586 / 15589
页数:4
相关论文
共 28 条
[1]   Murine T cells potently restrict human immunodeficiency virus infection [J].
Baumann, JG ;
Unutmaz, D ;
Miller, MD ;
Breun, SKJ ;
Grill, SM ;
Mirro, J ;
Littman, DR ;
Rein, A ;
KewalRamani, VN .
JOURNAL OF VIROLOGY, 2004, 78 (22) :12537-12547
[2]   Multiple blocks to human immunodeficiency virus type 1 replication in rodent cells [J].
Bieniasz, PD ;
Cullen, BR .
JOURNAL OF VIROLOGY, 2000, 74 (21) :9868-9877
[3]   Efficient assembly of an HIV-1/MLV Gag-chimeric virus in murine cells [J].
Chen, BK ;
Rousso, I ;
Shim, S ;
Kim, PS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (26) :15239-15244
[4]   HIV-1 Entry Cofactor: Functional cDNA Cloing of a Seven-Transmembrane, G protein-Coupled Receptor [J].
Feng, Yu ;
Broder, Christopher C. ;
Kennedy, Paul E. ;
Berger, Edward A. .
JOURNAL OF IMMUNOLOGY, 2011, 186 (11) :872-877
[5]   The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein [J].
Garber, ME ;
Wei, P ;
KewalRamani, VN ;
Mayall, TP ;
Herrmann, CH ;
Rice, AP ;
Littman, DR ;
Jones, KA .
GENES & DEVELOPMENT, 1998, 12 (22) :3512-3527
[6]  
Hatziioannou T, 2004, J VIROL, V78, P6005, DOI 10.1128/JVI.78.11.6005-6012.2004
[7]   Capsid-dependent and -independent postentry restriction of primate lentivirus tropism in rodent cells [J].
Hatziioannou, T ;
Cowan, S ;
Bieniasz, PD .
JOURNAL OF VIROLOGY, 2004, 78 (02) :1006-1011
[8]   Restriction of multiple divergent retroviruses by Lv1 and Ref1 [J].
Hatziioannou, T ;
Cowan, S ;
Goff, SP ;
Bieniasz, PD ;
Towers, GJ .
EMBO JOURNAL, 2003, 22 (03) :385-394
[9]   Ability of small animal cells to support the postintegration phase of human immunodeficiency virus type-1 replication [J].
Koito, A ;
Shigekane, H ;
Matsushita, S .
VIROLOGY, 2003, 305 (01) :181-191
[10]   A Rev-independent human immunodeficiency virus type 1 (HIV-1)-based vector that exploits a codon-optimized HIV-1 gag-pol gene [J].
Kotsopoulou, E ;
Kim, VN ;
Kingsman, AJ ;
Kingsman, SM ;
Mitrophanous, KA .
JOURNAL OF VIROLOGY, 2000, 74 (10) :4839-4852