Easy ambient sonic-spray ionization mass spectrometry for tissue imaging

被引：20

作者：

Alberici, Rosana M. ^{[1
]}

Vendramini, Pedro H. ^{[1
]}

Eberlin, Marcos N. ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP, Brazil

来源：

ANALYTICAL METHODS | 2017年 / 9卷 / 34期

基金：

巴西圣保罗研究基金会;

关键词：

DESORPTION ELECTROSPRAY-IONIZATION; ATMOSPHERIC-PRESSURE; LIPID ANALYSIS; BRAIN; DESI; MS;

D O I：

10.1039/c7ay00858a

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Two spray-based ambient ionization methods, desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI), have been successfully used to obtain tissue images of rat brain samples, for the first time, using both sources under their optimized conditions. Good ion images have been obtained by both techniques in MS and no significant difference was observed in the spray spot size. A comparative study between DESI and EASI on sensibility was also done and EASI was found to have similar or slightly higher sensitivity than DESI depending on the lipids interrogated (fatty acids, triacylglycerols and phospholipids). Thus, EASI can therefore be applied for tissue imaging particularly when the application of high voltage is impractical or undesirable.

引用

页码：5029 / 5036

页数：8

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