The Saccharomyces cerevisiae ORF YNR064c protein has characteristics of an 'orphaned' epoxide hydrolase

被引：16

作者：

Elfström, LT ^{[1
]}

Widersten, M ^{[1
]}

机构：

[1] Uppsala Univ, Biomed Ctr, Dept Biochem, SE-75123 Uppsala, Sweden

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1748卷 / 02期

关键词：

unidentified ORF; alpha/beta-hydrolase fold; Saccharomyces cerevisiae; heterologous expression;

D O I：

10.1016/j.bbapap.2005.01.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The open reading frame YNR064c in Saccharomyces cerevisiae encodes a protein tentatively assigned as similar to a bacterial dehalogenase. In this study we conclude that the YNR064c protein displays characteristics of an epoxide hydrolase belonging to the alpha/beta-hydrolase fold family of enzymes. Endogenous expression of the protein in S. cerevisiae was confirmed and a His-tagged variant of the protein was heterologously expressed in both Escherichia coli and Pichia pastoris for isolation and characterization. The YNR064c protein displayed low but reproducible epoxide hydrolase activity with racemic phenanthrene 9,10-oxide and trans- or cis-stilbene oxide. Phylogenetic analysis of related gene products found in various microorganisms suggested that the YNR064c protein is a member of a new subclass of alpha/beta-hydrolase fold enzymes. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：213 / 221

页数：9

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