K-562 cells lack MHC class II expression due to an alternatively spliced CIITA transcript with a truncated coding region

被引：10

作者：

Day, NE

Ugai, H

Yokoyama, KK

Ichiki, AT

机构：

[1] Univ Tennessee, Med Ctr, Dept Med Genet, Grad Sch Med, Knoxville, TN 37920 USA

[2] Tsukuba Inst, Tsukuba Sci City, Gene Engn Div, Dept Biol Syst,BioResource Ctr,RIKEN, Tsukuba, Ibaraki 3050074, Japan

来源：

LEUKEMIA RESEARCH | 2003年 / 27卷 / 11期

基金：

美国国家科学基金会;

关键词：

K-562; CIITA; MHC class II; CIITA promoter-IV; IFN-gamma-inducible;

D O I：

10.1016/S0145-2126(03)00072-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The focus of this study was to determine the functional capacity of class II transactivator (CIITA), a regulatory factor of major histocompatibility complex (MHC) class II genes, in K-562 cells. We show that CIITA mRNA is present in K-562 cells and the interferon-gamma (IFN-gamma)-inducible CIITA promoter-IV exhibits low levels of basal activity, which is greatly enhanced upon treatment with IFN-gamma. Further study revealed that the CIITA cDNA contains an insertion of genomic sequence, which introduces a stop codon. The truncated coding region of the CIITA transcript in K-562 cells provides a possible explanation for the absence of MHC class II molecules. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：1027 / 1038

页数：12

共 49 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] PMA-treated K-562 leukemia cells mediate a TH2-specific expansion of CD4+T cells in vitro [J].

Baker, EJ ;

Ichiki, AT ;

Hodge, JW ;

Sugantharaj, D ;

Bamberger, EG ;

Lozzio, CB .

LEUKEMIA RESEARCH, 2000, 24 (12) :1049-1057

[3] EMBRYONIC-FETAL ERYTHROID CHARACTERISTICS OF A HUMAN-LEUKEMIC CELL-LINE [J].

BENZ, EJ ;

MURNANE, MJ ;

TONKONOW, BL ;

BERMAN, BW ;

MAZUR, EM ;

CAVALLESCO, C ;

JENKO, T ;

SNYDER, EL ;

FORGET, BG ;

HOFFMAN, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3509-3513

[4]

Dong YS, 1999, J IMMUNOL, V162, P4731

[5]

DREW SI, 1977, BLOOD, V49, P715

[6] RFXAP, a novel subunit of the RFX DNA binding complex is mutated in MHC class II deficiency [J].

Durand, B ;

Sperisen, P ;

Emery, P ;

Barras, E ;

Zufferey, M ;

Mach, B ;

Reith, W .

EMBO JOURNAL, 1997, 16 (05) :1045-1055

[7]

GARSON D, 1985, EXP HEMATOL, V13, P885

[8]

Goodwin BL, 2001, CELL GROWTH DIFFER, V12, P327

[9] CIITA leucine-rich repeats control nuclear localization, in vivo recruitment to the major histocompatibility complex (MHC) class II enhanceosome, and MHC class II gene transactivation [J].

Hake, SB ;

Masternak, K ;

Kammerbauer, C ;

Janzen, C ;

Reith, W ;

Steimle, V .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (20) :7716-7725

[10] GTP binding by class II transactivator: Role in nuclear import [J].

Harton, JA ;

Cressman, DE ;

Chin, KC ;

Der, CJ ;

Ting, JPY .

SCIENCE, 1999, 285 (5432) :1402-1405

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