Quantification of Etheno-DNA adducts using liquid chromatography, on-line sample processing, and electrospray tandem mass spectrometry

被引:68
作者
Doerge, DR [1 ]
Churchwell, MI [1 ]
Fang, JL [1 ]
Beland, FA [1 ]
机构
[1] US FDA, Natl Ctr Toxicol Res, Div Biochem Toxicol, Jefferson, AR 72079 USA
关键词
D O I
10.1021/tx0001575
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Etheno-DNA adducts are promutagenic lesions present in normal animal and human tissues that are believed to be important in the etiology of cancer related to diet and lifestyle. A method has been developed for the quantification of trace levels of etheno-DNA adducts using on-line sample preparation coupled with liquid chromatography and electrospray tandem mass spectrometry. The use of automat ed solid-phase extraction and stable labeled internal standards permitted the robust determination of ethenodeoxyadenosine contained in crude DNA hydrolysates from untreated rodent and human tissues at levels on the order of one adduct in 10(8) normal nucleotides from 100 mug of DNA. Inherent analyte response and matrix interference made sensitivity for simultaneous determination of ethenodeoxycytidine approximately 5-fold lower. The method was applied to the analysis of liver DNA from untreated and urethane-treated B6C3F1 mice, untreated rat liver, human placenta, and several commercial DNA preparations. Some sources of potential artifactual formation of etheno-DNA adducts were investigated.
引用
收藏
页码:1259 / 1264
页数:6
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