Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence

被引：54

作者：

Fukuchi-Mizutani, M

Ishiguro, K

Nakayama, T

Utsunomiya, Y

Tanaka, Y

Kusumi, T

Ueda, T

机构：

[1] Suntory Ltd, Inst Fundamental Res, Shimamoto, Osaka 6188503, Japan

[2] Kobe Gakuin Univ, Fac Nutr, Nishi Ku, Kobe, Hyogo 65121, Japan

[3] Tohoku Univ, Dept Biochem & Engn, Aoba Ku, Sendai, Miyagi 9808579, Japan

来源：

PLANT SCIENCE | 2000年 / 160卷 / 01期

关键词：

Rosa hybrida; flower senescence; lipoxygenase; ethylene;

D O I：

10.1016/S0168-9452(00)00373-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlos1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rloxl lipoxygenase is involved in senescence of rose flowers. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：129 / 137

页数：9

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