Immunization with a single major histocompatibility complex class I-restricted cytotoxic T-lymphocyte recognition epitope of herpes simplex virus type 2 confers protective immunity
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Blaney, JE
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Blaney, JE
Nobusawa, E
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Nobusawa, E
Brehm, MA
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Brehm, MA
Bonneau, RH
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Bonneau, RH
Mylin, LM
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Mylin, LM
Fu, TM
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Fu, TM
Kawaoka, Y
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Kawaoka, Y
Tevethia, SS
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机构:Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Tevethia, SS
机构:
[1] Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
[2] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA
We have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (HSV-2) by selectively inducing an HSV-specific CD8(+) cytotoxic T-lymphocyte (CTL) response directed against a single major histocompatibility complex class I-restricted CTL recognition epitope. We generated a recombinant vaccinia virus (rVV-ES-gB498-505) which expresses the H-2K(b)-restricted, HSV-1/2-cross-reactive CTL recognition epitope, HSV glycoprotein B residues 498 to 505 (SSIEFARL) (gB498-505), fused to the adenovirus type 5 E3/19K endoplasmic reticulum insertion sequence (ES). Mucosal immunization of C57BL/6 mice with this recombinant vaccinia virus induced both a primary CTL response in the draining lymph nodes and a splenic memory CTL response directed against HSV gB498-505. To determine the ability of the gB498-505-specific memory CTL response to provide protection from HSV infection, immunized mice were challenged with a lethal dose of HSV-2 strain 186 by the intranasal (i.n.) route. Development of the gB498-505-specific CTL response conferred resistance in 60 to 75% of mice challenged with a lethal dose of HSV-2 and significantly reduced the levels of infectious virus in the brains and trigeminal ganglia of challenged mice. Finally, i.n. immunization of C57BL/6 mice with either a recombinant influenza virus or a recombinant vaccinia virus expressing HSV gB498-505 without the ES was also demonstrated to induce an HSV-specific CTL response and provide protection from HSV infection. This finding confirms that the induction of an HSV-specific CTL response directed against a single epitope is sufficient for conferring protective immunity to HSV. Our findings support the role of CD8(+) T cells in the control of HSV infection of the central nervous system and suggest the potential importance of eliciting HSV-specific mucosal CD8(+) CTL in HSV vaccine design.
机构:
PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USA
BONNEAU, RH
;
FU, TM
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PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USA
FU, TM
;
TEVETHIA, SS
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PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USA
机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
BONNEAU, RH
;
JENNINGS, SR
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机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
BONNEAU, RH
;
JENNINGS, SR
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机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
机构:
PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USA
BONNEAU, RH
;
FU, TM
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h-index: 0
机构:
PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USA
FU, TM
;
TEVETHIA, SS
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h-index: 0
机构:
PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USAPENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, DEPT MICROBIOL & IMMUNOL, HERSHEY, PA 17033 USA
机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
BONNEAU, RH
;
JENNINGS, SR
论文数: 0引用数: 0
h-index: 0
机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033
BONNEAU, RH
;
JENNINGS, SR
论文数: 0引用数: 0
h-index: 0
机构:
PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT MICROBIOL & IMMUNOL,HERSHEY,PA 17033