OC-2, a novel mammalian member of the ONECUT class of homeodomain transcription factors whose function in liver partially overlaps with that of hepatocyte nuclear factor-6

被引:91
作者
Jacquemin, P
Lannoy, VJ
Rousseau, GG
Lemaigre, FP
机构
[1] Univ Catholique Louvain, Sch Med, Hormone & Metab Res Unit, B-1200 Brussels, Belgium
[2] Christian de Duve Inst Cellular Pathol, B-1200 Brussels, Belgium
关键词
D O I
10.1074/jbc.274.5.2665
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6, are characterized by the presence of a single cut domain and by a peculiar homeodomain (Lannoy, V. J., Burglin, T. R., Rousseau, G. G., and Lemaigre, F. P. (1998) J. Biol. Chem, 273, 13552-13562), We report here the identification and characterization of human OC-2, the second mammalian member of this class. The OC-2 gene is located on human chromosome 18, The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. The amino acid sequence of OC-2 contains several regions of high similarity to HNF-6, The recognition properties of OC-2 for binding sites present in regulatory regions of liver-expressed genes differ from, but overlap with, those of HNF 6. Like HNF-6, OC-2 stimulates transcription of the hnf-3 beta gene in transient transfection experiments, suggesting that OC-2 participates in the network of transcription factors required for liver differentiation and metabolism.
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收藏
页码:2665 / 2671
页数:7
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