Conserved organization of the ILT/LIR gene family within the polymorphic human leukocyte receptor complex

被引:34
作者
Young, NT [1 ]
Canavez, F
Uhrberg, M
Shum, BP
Parham, P
机构
[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
关键词
genes; leukocyte receptor complex; LIR; ILT; polymorphism;
D O I
10.1007/s002510100332
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The human leukocyte receptor complex (LRC) at Chromosome 19q13.4 encodes Ig superfamily proteins which regulate the function of various hematopoietic cell types. We investigated characteristics of the Ig-like transcript (ILT)/leukocyte Ig-like receptor (LIR) group of LRC genes in comparison with the other major LRC loci encoding the killer cell Ig-like receptors (KIRs). In direct contrast to KIR genes, the ILT/LIR loci of ethnically diverse individuals did not display haplotypic variations in gene number. Investigation of gene expression identified novel cDNA sequences related to the ILT2/LIR1, ILT4/LIR2., ILT3/LIR5. and ILT7 loci. while phylogenetic analysis revealed two distinct lineages of ILT/ LIR genes. These two lineages differ in both the nature and extent of their sequence polymorphism. The presence of certain transcription factor-related motifs in the 5 ' untranslated region of ILT/LIR cDNAs correlates with the specific cell types in which particular ILT/LIR genes are expressed. Although extensive gene duplications and conversion events have apparently forged the LRC, our results indicate striking conservation in the organization of the ILT/ LIR genes when compared with the related and closely linked KIR genes. This suggests the evolutionary maintenance of a significant function consistent with the cellular distribution of the ILT/LIR proteins.
引用
收藏
页码:270 / 278
页数:9
相关论文
共 45 条
[1]   Genomic structure of PIR-B, the inhibitory member of the paired immunoglobulin-like receptor genes in mice [J].
Alley, TL ;
Cooper, MD ;
Chen, M ;
Kubagawa, H .
TISSUE ANTIGENS, 1998, 51 (03) :224-231
[2]  
Arm JP, 1997, J IMMUNOL, V159, P2342
[3]  
Baker E, 1995, CHROMOSOME RES, V3, P511
[4]   Complete sequence and gene map of a human major histocompatibility complex [J].
Beck, S ;
Geraghty, D ;
Inoko, H ;
Rowen, L ;
Aguado, B ;
Bahram, S ;
Campbell, RD ;
Forbes, SA ;
Guillaudeux, T ;
Hood, L ;
Horton, R ;
Janer, M ;
Jasoni, C ;
Madan, A ;
Milne, S ;
Neville, M ;
Oka, A ;
Qin, S ;
Ribas-Despuig, G ;
Rogers, J ;
Shiina, T ;
Spies, T ;
Tamiya, G ;
Tashiro, H ;
Trowsdale, J ;
Vu, Q ;
Williams, L ;
Yamazaki, M .
NATURE, 1999, 401 (6756) :921-923
[5]  
Borges L, 1997, J IMMUNOL, V159, P5192
[6]   Recruitment of tyrosine phosphatase HCP by the killer cell inhibitory receptor [J].
Burshtyn, DN ;
Scharenberg, AM ;
Wagtmann, N ;
Rajagopalan, S ;
Berrada, K ;
Yi, TL ;
Kinet, JP ;
Long, EO .
IMMUNITY, 1996, 4 (01) :77-85
[7]   The inhibitory receptor LIR-1 uses a common binding interaction to recognize class I MHC molecules and the viral homolog UL18 [J].
Chapman, TL ;
Heikema, AP ;
Bjorkman, PJ .
IMMUNITY, 1999, 11 (05) :603-613
[8]   Crystal structure and ligand binding properties of the D1D2 region of the inhibitory receptor LIR-1 (ILT2) [J].
Chapman, TL ;
Heikema, AP ;
West, AP ;
Bjorkman, PJ .
IMMUNITY, 2000, 13 (05) :727-736
[9]   Paternal monoallelic expression of the paired immunoglobulin-like receptors PIR-A and PIR-B [J].
Chen, CC ;
Hurez, V ;
Brockenbrough, JS ;
Kubagawa, H ;
Cooper, MD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) :6868-6872
[10]  
Colonna M, 1998, J IMMUNOL, V160, P3096