Suppression of ClC-3 channel expression reduces migration of nasopharyngeal carcinoma cells

被引：81

作者：

Mao, Jianwen ^{[2
,3
]}

Chen, Lixin ^{[1
]}

Xu, Bin ^{[2
,3
]}

Wang, Lijing ^{[2
,3
]}

Li, Hongzhi ^{[2
,3
]}

Guo, Jiao ^{[2
,3
]}

Li, Weidong ^{[2
,3
]}

Nie, Sihuai ^{[4
]}

Jacob, Tim J. C. ^{[5
]}

Wang, Liwei ^{[1
]}

机构：

[1] Jinan Univ, Coll Med, Guangzhou 510632, Peoples R China

[2] Guangdong Pharmaceut Univ, Inst Basic Med Sci, Guangzhou 510006, Peoples R China

[3] Guangdong Pharmaceut Univ, Dept Biol, Guangzhou 510006, Peoples R China

[4] Guangdong Med Coll, Cell Biol Lab, Zhanjiang 524023, Peoples R China

[5] Cardiff Univ, Cardiff Sch Biosci, Cardiff CF10 3US, Wales

来源：

BIOCHEMICAL PHARMACOLOGY | 2008年 / 75卷 / 09期

基金：

英国惠康基金;

关键词：

ClC-3; chloride channels; antisense oligonucleotide; cell migration; cell volume;

D O I：

10.1016/j.bcp.2008.01.008

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Recent studies suggest that chloride (Cl-) channels regulate tumor cell migration. In this report, we have used antisense oligonucleotides specific for ClC-3, the most likely molecular candidate for the volume-activated Cl- channel, to investigate the role of ClC-3 in the migration of nasopharyngeal carcinoma cells (CNE-2Z) in vitro. We found that suppression of ClC-3 expression inhibited the migration of CNE-2Z cells in a concentration-dependent manner. Whole-cell patch-clamp recordings and image analysis further demonstrated that ClC-3 suppression inhibited the volume-activated Cl- current (I-Cl,I-vol) and regulatory volume decrease (RVD) of CNE-2Z cells. The expression of ClC-3 positively correlated with cell migration, I-Cl,I-vol and RVD. These results strongly suggest that ClC-3 is a component or regulator of the volume-activated Cl- channel. ClC-3 may regulate CNE-2Z cell migration by modulating cell volume. ClC-3 may be a new target for cancer therapies. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：1706 / 1716

页数：11

共 42 条

[1] The breast cancer β4 integrin and endothelial human CLCA2 mediate lung metastasis [J].

Abdel-Ghany, M ;

Cheng, HC ;

Elble, RC ;

Pauli, BU .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (27) :25438-25446

[2] Identification of MCL1 as a novel target in neoplastic mast cells in systemic mastocytosis:: inhibition of mast cell survival by MCL1 antisense oligonucleotides and synergism with PKC412 [J].

Aichberger, Karl J. ;

Mayerhofer, Matthias ;

Gleixner, Karoline V. ;

Krauth, Maria-Theresa ;

Gruze, Alexander ;

Pickl, Winfried F. ;

Wacheck, Volker ;

Selzer, Edgar ;

Muellauer, Leonhard ;

Agis, Hermine ;

Sillaber, Christian ;

Valent, Peter .

BLOOD, 2007, 109 (07) :3031-3041

[3] Re-expression of detachment-inducible chloride channel mCLCA5 suppresses growth of metastatic breast cancer cells [J].

Beckley, JR ;

Pauli, BU ;

Elble, RC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (40) :41634-41641

[4] Cell cycle-dependent expression of volume-activated chloride currents in nasopharyngeal carcinoma cells [J].

Chen, LX ;

Wang, LW ;

Zhu, LY ;

Nie, SH ;

Zhang, J ;

Zhong, P ;

Cai, B ;

Luo, HB ;

Jacob, TJC .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2002, 283 (04) :C1313-C1323

[5] Association of intrinsic pICln with volume-activated Cl- current and volume regulation in a native epithelial cell [J].

Chen, LX ;

Wang, LW ;

Jacob, TJC .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1999, 276 (01) :C182-C192

[6] Molecular identification of a volume-regulated chloride channel [J].

Duan, D ;

Winter, C ;

Cowley, S ;

Hume, JR ;

Horowitz, B .

NATURE, 1997, 390 (6658) :417-421

[7] Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody [J].

Duan, DY ;

Zhong, JM ;

Hermoso, M ;

Satterwhite, CM ;

Rossow, CF ;

Hatton, WJ ;

Yamboliev, I ;

Horowitz, B ;

Hume, JR .

JOURNAL OF PHYSIOLOGY-LONDON, 2001, 531 (02) :437-444

[8]

Ehrengruber MU, 1996, J EXP BIOL, V199, P741

[9] Relative contribution of chloride channels and transporters to regulatory volume decrease in human glioma cells [J].

Ernest, NJ ;

Weaver, AK ;

Van Duyn, LB ;

Sontheimer, HW .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2005, 288 (06) :C1451-C1460

[10] The CIC-3Cl- channel in cell volume regulation, proliferation and apoptosis in vascular smooth muscle cells [J].

Guan, YY ;

Wang, GL ;

Zhou, JG .

TRENDS IN PHARMACOLOGICAL SCIENCES, 2006, 27 (06) :290-296

← 1 2 3 4 5 →