学术探索
学术期刊
学术作者
新闻热点
数据分析
智能评审

Down-regulation of the Wnt, estrogen receptor, insulin-like growth factor-I, and bone morphogenetic protein pathways in osteoblasts from rats with chronic spinal cord injury

被引：24
作者：
Jiang, Sheng-Dan [1 ]
Yan, Jun [1 ]
Jiang, Lei-Sheng [1 ]
Dai, Li-Yang [1 ]
机构：
[1] Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Orthopaed Surg, Shanghai 200092, Peoples R China
来源：
JOINT BONE SPINE | 2011年 / 78卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Osteoporosis; Spinal cord injury; Osteoblasts; Microarray analysis; BIOMECHANICAL PROPERTIES; GROWING RATS; SUBSTANCE-P; YOUNG-RATS; METABOLISM; OSTEOCLASTOGENESIS; RESORPTION; SKELETON; MASS;
D O I：
10.1016/j.jbspin.2010.12.005
中图分类号：
R5 [内科学];
学科分类号：
100201 [内科学];
摘要：
Objectives: To investigate the anabolic response of osteoblasts to chronic spinal cord injury and to identify potential signaling pathways that are associated with the osteogenic response after spinal cord injury by using in-house microarray analyses in osteoblasts. Methods: Ten young male Sprague-Dawley rats were randomized into spinal cord injury (SCI) and SHAM groups. The tibiae were assessed for DXA and bone histomorphometry, and osteoblasts from femora were used for microarray analysis. Results: SCI rats showed lower BMD and deteriorated microstructure in the proximal tibiae as compared with SHAM rats. The Wnt, BMP/TGF, estrogen receptor (ER), and IGF-I pathways were down-regulated in osteoblasts from spinal cord-injured rats. Conclusion: Down-regulation of the Wnt, BMP/TGF, ER, and growth hormone/IGF-I pathways is associated with decreased bone formation after spinal cord injury. (C) 2011 Societe francaise de rhumatologie. Published by Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：488 / 492
页数：5
相关论文
共 21 条
[1]
Bone morphogenetic proteins, their antagonists, and the skeleton [J].
Canalis, E ;
Economides, AN ;
Gazzerro, E .
ENDOCRINE REVIEWS, 2003, 24 (02) :218-235
[2]
Effect of alendronate on bone mineral density in spinal cord injury patients: a pilot study [J].
de Brito, CMM ;
Battistella, LR ;
Saito, ET ;
Sakamoto, H .
SPINAL CORD, 2005, 43 (06) :341-348
[3]
Development of sensory innervation in rat tibia: co-localization of CGRP and substance P with growth-associated protein 43 (GAP-43) [J].
Gajda, M ;
Litwin, JA ;
Cichocki, T ;
Timmermans, JP ;
Adriaensen, D .
JOURNAL OF ANATOMY, 2005, 207 (02) :135-144
[4]
Bone loss at the os calcis compared with bone loss at the knee in individuals with spinal cord injury [J].
Garland, DE ;
Adkins, RH ;
Scott, M ;
Singh, H ;
Massih, M ;
Stewart, C .
JOURNAL OF SPINAL CORD MEDICINE, 2004, 27 (03) :207-211
[5]
Twisted gastrulation, a bone morphogenetic protein agonist/antagonist, is not required for post-natal skeletal function [J].
Gazzerro, Elisabetta ;
Deregowski, Valerie ;
Stadmeyer, Lisa ;
Gale, Nickolas W. ;
Economides, Aris N. ;
Canalis, Ernesto .
BONE, 2006, 39 (06) :1252-1260
[6]
Neuronal regulation of bone metabolism and anabolism: Calcitonin gene-related peptide-, substance P-, and tyrosine hydroxylase-containing nerves and the bone [J].
Imai, S ;
Matsusue, Y .
MICROSCOPY RESEARCH AND TECHNIQUE, 2002, 58 (02) :61-69
[7]
Changes in bone mass, bone structure, bone biomechanical properties, and bone metabolism after spinal cord injury: A 6-month longitudinal study in growing rats [J].
Jiang, S. -D. ;
Jiang, L. -S. ;
Dai, L. -Y. .
CALCIFIED TISSUE INTERNATIONAL, 2007, 80 (03) :167-175
[8]
Effects of spinal cord injury on osteoblastogenesis, osteoclastogenesis and gene expression profiling in osteoblasts in young rats [J].
Jiang, S. -D. ;
Jiang, L. -S. ;
Dai, L. -Y. .
OSTEOPOROSIS INTERNATIONAL, 2007, 18 (03) :339-349
[9]
Spinal cord injury causes more damage to bone mass, bone structure, biomechanical properties and bone metabolism than sciatic neurectomy in young rats [J].
Jiang, S. -D. ;
Jiang, L. -S. ;
Dai, L. -Y. .
OSTEOPOROSIS INTERNATIONAL, 2006, 17 (10) :1552-1561
[10]
Osteoporosis after spinal cord injury [J].
Jiang, SD ;
Dai, LY ;
Jiang, LS .
OSTEOPOROSIS INTERNATIONAL, 2006, 17 (02) :180-192
123