Detection of rotavirus in sewage and creek water: Efficiency of the concentration method

被引：23

作者：

Mehnert, DU

Stewien, KE

Harsi, CM

Queiroz, APS

Candeias, JMG

Candeias, JAN

机构：

[1] Departamento de Microbiologia, Inst. de Cie. Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1997年 / 92卷 / 01期

关键词：

rotavirus; sewage; virus concentration; direct immunoperoxidase;

D O I：

10.1590/S0074-02761997000100020

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method a mean recovery of 81% +/- 7.5 of the SA-11 virus, was achieved.

引用

页码：97 / 100

页数：4

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