Detection of rotavirus in sewage and creek water: Efficiency of the concentration method

被引:23
作者
Mehnert, DU
Stewien, KE
Harsi, CM
Queiroz, APS
Candeias, JMG
Candeias, JAN
机构
[1] Departamento de Microbiologia, Inst. de Cie. Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1997年 / 92卷 / 01期
关键词
rotavirus; sewage; virus concentration; direct immunoperoxidase;
D O I
10.1590/S0074-02761997000100020
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method a mean recovery of 81% +/- 7.5 of the SA-11 virus, was achieved.
引用
收藏
页码:97 / 100
页数:4
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