In vitro and in vivo reconstitution of the cadherin-catenin-actin complex from Caenorhabditis elegans

被引：69

作者：

Kwiatkowski, Adam V. ^{[1
]}

Maiden, Stephanie L. ^{[2
,3
]}

Pokutta, Sabine ^{[4
]}

Choi, Hee-Jung ^{[4
]}

Benjamin, Jacqueline M. ^{[1
]}

Lynch, Allison M. ^{[5
]}

Nelson, W. James ^{[1
,6
]}

Weis, William I. ^{[4
,6
]}

Hardin, Jeff ^{[2
,3
]}

机构：

[1] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[2] Univ Wisconsin, Mol & Cellular Pharmacol Program, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA

[4] Stanford Univ, Dept Biol Struct, Sch Med, Stanford, CA 94305 USA

[5] Univ Wisconsin, Genet Program, Madison, WI 53706 USA

[6] Stanford Univ, Sch Med, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 33期

基金：

美国国家卫生研究院;

关键词：

cell-cell adhesion; HMP-1; HMP-2; HMR-1; CELL-CELL ADHESION; ALPHA-CATENIN; CRYSTAL-STRUCTURE; BETA-CATENIN; F-ACTIN; INTRAMOLECULAR ASSOCIATION; ADHERENS JUNCTIONS; TAIL DOMAINS; VINCULIN; BINDING;

D O I：

10.1073/pnas.1007349107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ternary complex of cadherin, beta-catenin, and alpha-catenin regulates actin-dependent cell-cell adhesion. alpha-Catenin can bind beta-catenin and F-actin, but in mammals alpha-catenin either binds beta-catenin as a monomer or F-actin as a homodimer. It is not known if this conformational regulation of alpha-catenin is evolutionarily conserved. The Caenorhabditis elegans alpha-catenin homolog HMP-1 is essential for actin-dependent epidermal enclosure and embryo elongation. Here we show that HMP-1 is a monomer with a functional C-terminal F-actin binding domain. However, neither full-length HMP-1 nor a ternary complex ofHMP-1-HMP-2(beta-catenin)-HMR-1(cadherin)bind F-actin in vitro, suggesting that HMP-1 is auto-inhibited. Truncation of either the F-actin or HMP-2 binding domain of HMP-1 disrupts C. elegans development, indicating that HMP-1 must be able to bind F-actin and HMP-2 to function in vivo. Our study defines evolutionarily conserved properties of alpha-catenin and suggests that multiple mechanisms regulate alpha-catenin binding to F-actin.

引用

页码：14591 / 14596

页数：6

共 32 条

[1] EPLIN mediates linkage of the cadherin-catenin complex to F-actin and stabilizes the circumferential actin belt [J].