Differential binding properties of B7-H1 and B7-DC to programmed death-1

被引：197

作者：

Youngnak, P

Kozono, Y

Kozono, H

Iwai, H

Otsuki, N

Jin, H

Omura, K

Yagita, H

Pardoll, DM

Chen, LP

Azuma, M

机构：

[1] Tokyo Med & Dent Univ, Grad Sch, Dept Mol Immunol, Bunkyo Ku, Tokyo 1138549, Japan

[2] Tokyo Med & Dent Univ, Grad Sch, Dept Oral Surg, Bunkyo Ku, Tokyo 1138549, Japan

[3] Sci Univ Tokyo, Res Inst Biol Sci, Div Biosignaling, Noda, Chiba 2780022, Japan

[4] Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo 1138421, Japan

[5] Johns Hopkins Univ, Sch Med, Sidney Kimmel Canc Ctr, Baltimore, MD 21231 USA

[6] Mayo Grad & Med Sch, Mayo Clin, Dept Immunol, Rochester, MN 55905 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 307卷 / 03期

关键词：

PD-1; ligands; costimulatory molecule; relative affinity; surface plasmon resonance;

D O I：

10.1016/S0006-291X(03)01257-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble 1g fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：672 / 677

页数：6

共 40 条

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