Carbohydrate fluxes into alginate biosynthesis in Azotobacter vinelandii NCIB 8789: NMR investigations of the triose pools

被引:36
作者
Beale, JM
Foster, JL
机构
[1] Div. of Med. and Nat. Prod. Chem., College of Pharmacy, University of Texas at Austin, Austin
关键词
D O I
10.1021/bi951922v
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The metabolite flux of carbohydrates through primary catabolism and into hexose resynthesis has been investigated for alginic acid biosynthesis in Azotobacter vinelandii. To do these studies, we fed the microorganism a variety of C-13-labeled glucose precursors, including [U-C-13(6)]glucose. The incorporations of the precursors were determined by 1D C-13-NMR, 2D C-13-DQF-COSY, and inverse triple-quantum correlation experiments. The results clearly show that the entire catabolism of hexose is through the Entner-Doudoroff (E-D) pathway and that the triose pools are in equilibrium. Reentry into gluconeogenesis prior to alginate synthesis occurs totally from the glyceraldehyde 3-phosphate generated by the E-D pathway. The obligatory intermediacy of triose intermediates in alginate biosynthesis was proved. The experiments and results presented in this paper constitute a new method for distinguishing the E-D pathway from glycolysis in bacteria.
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收藏
页码:4492 / 4501
页数:10
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