Perichromatin fibrils as early markers of transcriptional alterations

被引：31

作者：

Biggiogera, Marco ^{[1
,2
]}

Cisterna, Barbara ^{[1
,2
]}

Spedito, Alessandro ^{[1
,2
]}

Vecchio, Lorella ^{[1
,2
]}

Malatesta, Manuela ^{[3
]}

机构：

[1] Univ Pavia, Lab Biol Cellulare & Neurobiol, Dipartimento Biol Anim, I-27100 Pavia, Italy

[2] CNR, Ist Genet Mol, I-27100 Pavia, Italy

[3] Univ Verona, Sez Anat & Istol, Dipartimento Sci Morfol Biomed, I-37100 Verona, Italy

来源：

DIFFERENTIATION | 2008年 / 76卷 / 01期

关键词：

transcription; perichromatin fibrils; electron microscopy; cell nucleus; ribonucleoproteins;

D O I：

10.1111/j.1432-0436.2007.00211.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Perichromatin fibrils represent the morphological expression of transcription and co-transcriptional processing of pre-mRNA. They can be considered, hence, an example of work in progress. High resolution techniques such as electron microscopy demonstrate that perichromatin fibrils play a role as early markers of transcriptional alterations. In this paper, we review some experimental and physiological conditions impairing or modulating transcription as well as their effects on perichromatin fibrils. In all the situations reported, perichromatin fibrils show modifications in their amount and/or their associated proteins. Their movements are also affected, as well as their export or their intra-nuclear storage forms. Perichromatin fibrils therefore represent highly sensitive markers not only for monitoring transcriptional and processing rate but also for identifying the maturation level of pre-mRNA/mRNA occurring in the cell nucleus and the functional correlation with the cellular metabolic state.

引用

页码：57 / 65

页数：9

共 87 条

[1] Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks [J].