A "Mix and read" assay for insulin using fluorometric microvolume assay technology

被引：4

作者：

Toney, JH

Ogawa, A

Blair, M

Park, YW

机构：

[1] Merck Res Labs, Dept Microbial Biochem, Rahway, NJ 07065 USA

[2] Merck Res Labs, Metab Disorders Diabet, Rahway, NJ 07065 USA

来源：

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES | 2003年 / 1卷 / 04期

关键词：

D O I：

10.1089/154065803322302772

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel, "mix and read" immunoassay for insulin in biological samples using FMAT. Current commercial assays for insulin require multiple washing steps and can be expensive. The insulin assay described is a simple two-step, time-saving assay and amenable to robotics. The linear response for the fluorometric signal is comparable to that observed using classical ELISA and RIA. A series of mouse plasma samples were tested for insulin levels and yielded results comparable to that measured using a commercial ELISA for insulin.

引用

收藏

页码：521 / 525

页数：5

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