Evidence of Borna disease virus genome detection in French domestic animals and in foxes (Vulpes vulpes)

被引：23

作者：

Dauphin, G ^{[1
]}

Legay, V ^{[1
]}

Sailleau, C ^{[1
]}

Smondack, S ^{[1
]}

Hammoumi, S ^{[1
]}

Zientara, S ^{[1
]}

机构：

[1] AFSSA Alfort, F-94703 Maisons Alfort, France

来源：

JOURNAL OF GENERAL VIROLOGY | 2001年 / 82卷

关键词：

D O I：

10.1099/0022-1317-82-9-2199

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Borna disease virus (BDV) is an enveloped, nonsegmented negative-stranded RNA virus which belongs to the Bornaviridae family. BDV is an aetiological agent of encephalitis in horses, sheep and several other vertebrate species. In order to extend our knowledge about the presence of BDV in France, a study based on BDV RNA detection by RT-nested-PCR was done with 196 animal tissues: 171 brain samples collected from different animal species (75 horses, 59 foxes, 31 cattle, 4 dogs, 1 sheep, 1 roe deer) and 25 horse blood samples. An RNA internal standard molecule was constructed and was co-amplified with the test template. This study reports the first detection of BDV RNA in France in 10 brain samples collected from horses, foxes and cattle, and from 14 horse blood samples. Detection of the BDV genome in the brains of six red foxes is the first evidence of BDV infection in this species.

引用

页码：2199 / 2204

页数：6

共 48 条

[1] Varied prevalence of Borna disease virus infection in Arabic, thoroughbred and their cross-bred horses in Iran [J].