A transient expression system to assay putative antifungal genes on powdery mildew infected barley leaves

被引：34

作者：

Nielsen, K

Olsen, O

Oliver, R

机构：

[1] Carlsberg Lab, Dept Physiol, DK-2500 Copenhagen, Denmark

[2] Carlsberg Res Lab, DK-2500 Copenhagen, Denmark

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 1999年 / 54卷 / 1-2期

关键词：

D O I：

10.1006/pmpp.1998.0184

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A transient expression system has been established to assay the regulation and impact of putative antifungal proteins at the single cell level in barley (Hordeum vulgare L.) epidermal leaf cells infected with powdery mildew (Erysiphe graminis f.sp. hordei). The system combines biolistic transformation with direct comparison of the fungal attack in transfected and wild type cells. Many epidermal cells showed detectable expression of marker genes including a novel improved version of green fluorescent protein (GFP). The marker was detectable for at least 10 days during which individual host cells supported an unaffected asexual life-cycle of the pathogen. (C) 1999 Academic Press.

引用

页码：1 / 12

页数：12

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[1] STRUCTURE AND TRANSCRIPTION OF THE NOPALINE SYNTHASE GENE REGION OF T-DNA [J].