Detection of cellular cytokine mRNA expression during orf virus infection in sheep:: differential interferon-γ mRNA expression by cells in primary versus reinfection skin lesions

被引:30
作者
Anderson, IE [1 ]
Reid, HW [1 ]
Nettleton, PF [1 ]
McInnes, CJ [1 ]
Haig, DM [1 ]
机构
[1] Int Res Ctr, Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland
关键词
parapoxvirus; orf virus; immunity; skin; IFN-gamma; IL-4; TNF-alpha; in situ hybridization;
D O I
10.1016/S0165-2427(01)00388-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In sheep infected with the parapoxvirus orf virus, primary infection orf skin lesions developed and resolved within 8 weeks. Reinfection lesions were smaller and resolved within 3 weeks. The host response in the skin was characterized by an accumulation of neutrophils, dendritic cells, CD4(+) T cells, CD8(+) T cells, B cells and T19(+) gamma delta T cells. The magnitude of this accumulation paralleled orf virus replication in the skin. In situ hybridization was used to detect cells expressing interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4 (IL-4) mRNAs in orf skin. Cells expressing IL-4 mRNA were not detected at any time after infection. Cells expressing IFN-gamma mRNA were detected after reinfection but not after primary infection. Cells expressing TNF-alpha mRNA included epidermal cells, vascular endothelium and uncharacterized cells that increased more rapidly in the skin after reinfection compared to primary infection. The results are consistent with a prominent role for IFN-gamma in the host immune response controlling the severity of the disease. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:161 / 176
页数:16
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