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Characterization of three Ixodes scapularis cDNAs protective against tick infestations

被引：83
作者：
Almazán, C
Blas-Machado, U
Kocan, KM
Yoshioka, JH
Blouin, EF
Mangold, AJ
de la Fuente, J [1 ]
机构：
[1] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
[2] Oklahoma State Univ, Ctr Vet Hlth Sci, Diagnost Lab, Stillwater, OK 74078 USA
[3] Estac Expt Agropecuaria Rafaela, Inst Nacl Tecnol Agropecuaria, Rafaela, Santa Fe, Argentina
[4] UCLM, JCCM, CSIC, Inst Invest Recursos Cinegeticos IREC, Ciudad Real 13005, Spain
来源：
VACCINE | 2005年 / 23卷 / 35期
基金：
美国国家卫生研究院;
关键词：
tick; vaccine; expression library immunization; EST; recombinant protein;
D O I：
10.1016/j.vaccine.2005.04.012
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
cDNA expression library immunization (ELI) and analysis of expressed sequenced tags (EST) in a mouse model of tick infestations was used to identified cDNA clones that affected I. scapularis. Three protective antigens against larval tick infestations, 4178, with homology to a nucleotidase, and 4D8 and 4E6 of unknown function, were selected for further characterization. All three antigens were expressed in all L scapularis stages and localized in adult tick tissues. 4D8 was shown to be conserved in six other tick species. Based on immunization trials with synthetic polypeptides against larvae and nymphs and on artificial feeding experiments of adults, these antigens, especially 4D8, appear to be good candidates for continued development of a vaccine for control of tick infestations and may be useful in a formulation to target multiple species of ticks. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4403 / 4416
页数：14
相关论文
共 46 条
[1]  
Ada Gordon, 2003, Expert Opin Emerg Drugs, V8, P27, DOI 10.1517/eoed.8.1.27.21025
[2]   Identification of protective antigens for the control of Ixodes scapularis infestations using cDNA expression library immunization [J].
Almazán, C ;
Kocan, KM ;
Bergman, DK ;
Garcia-Garcia, JC ;
Blouin, EF ;
de la Fuente, J .
VACCINE, 2003, 21 (13-14) :1492-1501
[3]  
Almazan C., 2003, Gene Therapy and Molecular Biology, V7, P43
[4]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[5]   PHYLOGENY OF HARD-TICK AND SOFT-TICK TAXA (ACARI, IXODIDA) BASED ON MITOCHONDRIAL 16S RDNA SEQUENCES [J].
BLACK, WC ;
PIESMAN, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :10034-10038
[6]   Characterization of genetic diversity in Dermacentor andersoni (Acari: Ixodidae) with body size and weight polymorphism [J].
de la Fuente, J ;
Almazán, C ;
Van den Bussche, RA ;
Bowman, J ;
Yoshioka, JH ;
Kocan, KM .
EXPERIMENTAL PARASITOLOGY, 2005, 109 (01) :16-26
[7]   Field studies and cost-effectiveness analysis of vaccination with Gavac™ against the cattle tick Boophilus microplus [J].
de la Fuente, J ;
Rodríguez, M ;
Redondo, M ;
Montero, C ;
García-García, JC ;
Méndez, L ;
Serrano, E ;
Valdés, M ;
Enriquez, A ;
Canales, M ;
Ramos, E ;
Boué, O ;
Machado, H ;
Lleonart, R ;
de Armas, CA ;
Rey, S ;
Rodríguez, JL ;
Artiles, M ;
García, L .
VACCINE, 1998, 16 (04) :366-373
[8]   Differential adhesion of major surface proteins 1a and 1b of the ehrlichial cattle pathogen Anaplasma marginale to bovine erythrocytes and tick cells [J].
de la Fuente, J ;
Garcia-Garcia, JC ;
Blouin, EF ;
Kocan, KM .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2001, 31 (02) :145-153
[9]  
de la Fuente Jose, 2003, Expert Rev Vaccines, V2, P583
[10]   Predicting subcellular localization of proteins based on their N-terminal amino acid sequence [J].
Emanuelsson, O ;
Nielsen, H ;
Brunak, S ;
von Heijne, G .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (04) :1005-1016
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