Molecular diagnosis of human papillomavirus (HPV) infections

被引：213

作者：

Molijn, A ^{[1
]}

Meter, B ^{[1
]}

Quint, W ^{[1
]}

van Doorn, LJ ^{[1
]}

机构：

[1] DDL, NL-2275 CX Voorburg, Netherlands

来源：

JOURNAL OF CLINICAL VIROLOGY | 2005年 / 32卷

关键词：

HPV-DNA; molecular diagnostics; genotyping; type-specific PCR; broad-spectrum PCR; reverse hybridization;

D O I：

10.1016/j.jcv.2004.12.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human papillomaviruses (HPVs) comprise more than 100 genotypes. The mucosal types can be divided into high-risk and low-risk (LR) types depending on the associated disease risk. HPV infection is mainly diagnosed by molecular methods, since reliable serological tools are not available and culture of the virus is not possible. Accurate molecular diagnostic techniques that can be used to inform patient management and follow-up after treatment are now available for detection and identification of HPV. The diagnosis of HPV infections in patients at risk of disease in a clinical setting requires a different approach from that used for epidemiological studies, vaccination trials and natural history studies. This review describes the different molecular methods available for HPV detection and genotyping and their possible clinical utility. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：S43 / S51

页数：9

共 86 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].