Monolayers of derivatized poly(L-lysine)-grafted poly(ethylene glycol) on metal oxides as a class of biomolecular interfaces

被引：126

作者：

Ruiz-Taylor, LA ^{[1
]}

Martin, TL ^{[1
]}

Zaugg, FG ^{[1
]}

Witte, K ^{[1
]}

Indermuhle, P ^{[1
]}

Nock, S ^{[1
]}

Wagner, P ^{[1
]}

机构：

[1] Zyomyx Inc, Hayward, CA 94545 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 03期

关键词：

D O I：

10.1073/pnas.98.3.852

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly(L-lysine)-grafted poly(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-derivatized poly(L-lysine)-grafted poly(ethylene glycol) copolymers, PLL-9[(PEGm)((1-x)) (PEG-biotin)(x)], where x varies from 0 to 1. Monolayers were produced on titanium dioxide substrates and characterized by x-ray photoelectron spectroscopy. The specific biorecognition properties of these biotinylated surfaces were investigated with the use of radiolabeled streptavidin alone and within complex protein mixtures. The PLL-g-PEG-biotin monolayers specifically capture streptavidin, even from a complex protein mixture, while still preventing nonspecific adsorption of other proteins. This streptavidin layer can subsequently capture biotinylated proteins. Finally, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this class of biomolecular interfaces for applications based on protein patterning.

引用

页码：852 / 857

页数：6

共 31 条

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