Cell death/proliferation and alterations in glial morphology contribute to changes in diffusivity in the rat hippocampus after hypoxia-ischemia

被引:54
作者
Anderova, Miroslava [1 ,2 ,3 ,4 ]
Vorisek, Ivan [2 ,3 ,4 ,5 ]
Pivonkova, Helena [2 ,3 ,4 ]
Benesova, Jana [2 ]
Vargova, Lydia [2 ,3 ,4 ]
Cicanic, Michal [2 ,3 ,4 ]
Chvatal, Alexandr [2 ,3 ,4 ]
Sykova, Eva [2 ,3 ,4 ,5 ]
机构
[1] Acad Sci Czech Republic, Inst Expt Med, Dept Cellular Neurophysiol, Neurobiol Lab,Vvi, Prague 14220 4, Czech Republic
[2] Acad Sci Czech Republic, Dept Neurosci, Prague 14220 4, Czech Republic
[3] Charles Univ Prague, Fac Med 2, Dept Neurosci, Prague, Czech Republic
[4] Charles Univ Prague, Fac Med 2, Ctr Cell Therapy & Tissue Repair, Prague, Czech Republic
[5] Inst Clin & Expt Med, Dept Diagnost & Intervent Radiol, Prague, Czech Republic
关键词
astrocytes; extracellular volume; microglia; neurons; NG2-glia; tortuosity; water diffusion; EXTRACELLULAR-SPACE; IN-SITU; CEREBRAL-ISCHEMIA; NEURONAL DAMAGE; SPINAL-CORD; BRAIN; INJURY; ASTROCYTES; CORTEX; PROLIFERATION;
D O I
10.1038/jcbfm.2010.168
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To understand the structural alterations that underlie early and late changes in hippocampal diffusivity after hypoxia/ischemia (H/I), the changes in apparent diffusion coefficient of water (ADC(W)) were studied in 8-week-old rats after H/I using diffusion-weighted magnetic resonance imaging (DW-MRI). In the hippocampal CA1 region, ADC(W) analyses were performed during 6 months of reperfusion and compared with alterations in cell number/cell-type composition, glial morphology, and extracellular space (ECS) diffusion parameters obtained by the real-time iontophoretic method. In the early phases of reperfusion (1 to 3 days) neuronal cell death, glial proliferation, and developing gliosis were accompanied by an ADC(W) decrease and tortuosity increase. Interestingly, ECS volume fraction was decreased only first day after H/I. In the late phases of reperfusion (starting 1 month after H/I), when the CA1 region consisted mainly of microglia, astrocytes, and NG2-glia with markedly altered morphology, ADC(W), ECS volume fraction and tortuosity were increased. Three-dimensional confocal morphometry revealed enlarged astrocytes and shrunken NG2-glia, and in both the contribution of cell soma/processes to total cell volume was markedly increased/decreased. In summary, the ADC(W) increase in the CA1 region underlain by altered cellular composition and glial morphology suggests that considerable changes in extracellular signal transmission might occur in the late phases of reperfusion after H/I. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 894-907; doi:10.1038/jcbfm.2010.168; published online 29 September 2010
引用
收藏
页码:894 / 907
页数:14
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