PCR detection and analysis of the free-living amoeba Naegleria in hot springs in Yellowstone and Grand Teton National Parks

被引：61

作者：

Sheehan, KB

Fagg, JA

Ferris, MJ

Henson, JM

机构：

[1] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA

[2] Montana State Univ, Thermal Biol Inst, Bozeman, MT 59717 USA

[3] Res Inst Children, New Orleans, LA 70118 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 10期

关键词：

D O I：

10.1128/AEM.69.10.5914-5918.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Free-living thermotolerant amoebae pose a significant health risk to people who soak and swim in habitats suitable for their growth, such as hot springs. In this survey of 23 different hot springs in Yellowstone and Grand Teton National Parks, we used PCR with primer sets specific for Naegleria to detect three sequence types that represent species not previously described, as well as a fourth sequence type identified as the pathogen Naegleria fowleri.

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页码：5914 / 5918

页数：5

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